Humanin Peptide
Humanin Peptide is a 24-amino-acid mitochondrial-derived peptide (MDP) encoded within the 16S ribosomal RNA gene of the mitochondrial genome. In biochemical and cellular assays, Humanin Peptide functions as an endogenous cytoprotective factor, interacting with multi-subunit membrane receptor complexes (comprising WSX-1, CNTFR, and gp130) as well as intracellular target proteins like Bax and IGFBP-3. Laboratory evaluations predominantly investigate Humanin Peptide for its role in suppressing mitochondrial ROS production, preventing apoptotic cascades, and preserving cellular bioenergetics under induced stress conditions.
Humanin Peptide | Complete Overview
Beyond its fundamental anti-apoptotic activity, research models utilizing Humanin demonstrate significant regulation over metabolic stress pathways and insulin sensitivity markers at the cellular level. In comparative cellular studies, Humanin is frequently evaluated alongside other mitochondrial-derived peptides (such as MOTS-c) to map downstream STAT3 phosphorylation and evaluate organelle-to-nucleus retrograde signaling dynamics. This dual capacity to protect mitochondrial membrane integrity while modulating metabolic homeostatic pathways establishes Humanin Peptide as an indispensable reference molecule for complex in vitro aging, neuroprotection, and metabolic disease assays.
Humanin Peptide | Scientific Information
Solubilization Guidance: Humanin Peptide demonstrates solid aqueous solubility. Reconstitute the lyophilized cake using sterile bacteriostatic water, sterile water, or phosphate-buffered saline (PBS, pH 7.4). For high-concentration stock preparations ($>2.0\text{ mg/mL}$), adding a small quantity of $0.1\%$ dilute acetic acid can aid complete dissolution.
Handling Method: Allow the vial to equilibrate to room temperature before reconstituted liquid vehicle is introduced along the inner glass wall. Rotate gently until completely dissolved. Avoid mechanical vortexing or sonicating to prevent shearing of the peptide sequence.
Preservation & Storage Conditions
Lyophilized Matrix: Store unopened dry powder desiccated at $-20^\circ\text{C}$ to $-80^\circ\text{C}$ for up to 24 months.
Reconstituted Phase: Refrigerate hydrated stock solutions at $2^\circ\text{C}$ to $8^\circ\text{C}$ for short-term evaluation (up to 14 days). For extended multi-month analytical protocols, aliquot into single-use micro-vials and store at $-80^\circ\text{C}$ to eliminate degradation from repeated freeze-thaw cycles.
Humanin Peptide | Educational Resource
High-Performance Liquid Chromatography (HPLC): Confirms $\ge 99.0\%$ sequence purity and rules out truncated residual peptides.
Liquid Chromatography-Mass Spectrometry (LC-MS): Validates precise molecular weight ($2687.2\text{ g/mol}$) and structural sequence identity.
Compliance Statement: Supplied exclusively as an analytical reference standard for in vitro laboratory research, biochemical testing, and non-clinical analytical assays.
